Polyethylenimine (PEI) Transfection Protocol Optimization: Dos and Don’ts for High Efficiency
Efficient gene delivery is essential for advancing cell and gene therapies. Polyethylenimine (PEI) transfection has become a widely adopted method due to its high DNA delivery efficiency, cost-effectiveness, and compatibility with multiple cell lines. Unlike lipid-based reagents, PEI offers scalability from early-stage research to large-scale commercial manufacturing, making it a practical choice for both discovery and production workflows.
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